What is 10X Tris-EDTA Buffer used for?

10X Tris-EDTA Buffer is frequently used in molecular biology applications, particularly in DNA and RNA extraction, purification, and electrophoresis. The Tris component maintains a stable pH, which is essential for enzyme reactions, while EDTA chelates divalent metal ions, protecting nucleic acids from degradation by nucleases. Many laboratories utilize this buffer in assays involving DNA cloning, PCR, and other genomic analyses.

How do you prepare a working solution from the 10X Tris-EDTA Buffer?

To prepare a working solution, you need to dilute the 10X Tris-EDTA Buffer to a 1X concentration by combining one part of the buffer with nine parts of distilled water. This is essential as using the buffer at its original concentration could lead to unintended results in your experiments. For example, in PCR reactions, ensuring correct buffer concentration can significantly influence amplification efficiency and specificity.

What are the storage recommendations for 10X Tris-EDTA Buffer?

10X Tris-EDTA Buffer should be stored at room temperature, away from light and moisture to maintain its stability. It is advisable to keep it tightly sealed in its original container. Proper storage ensures the buffer remains effective for a variety of applications, such as gel electrophoresis or sample preparation, allowing for consistent and reliable results in experiments.

Can I use 10X Tris-EDTA Buffer for RNA work?

Yes, 10X Tris-EDTA Buffer is safe for use in RNA applications, as its components do not inhibit enzymatic reactions involving RNA. The buffer’s ability to chelate metal ions makes it particularly useful for protecting RNA from degradation. In experiments such as RT-PCR or Northern blotting, using this buffer ensures that the RNA samples remain intact and viable for downstream applications.

Is the pH of Tris-EDTA Buffer critical?

Yes, the pH of Tris-EDTA Buffer is critical, as many biological reactions are pH-sensitive. The 10X Tris-EDTA Buffer has a pH of 8, which is optimal for most enzymatic reactions involving nucleic acids. This specific pH helps in maintaining the stability of DNA and RNA during various procedures, such as extraction or electrophoresis, thus enhancing the performance and accuracy of the experimental results.

What is the role of EDTA in the 10X Tris-EDTA Buffer?

EDTA (Ethylenediaminetetraacetic acid) plays a crucial role in the 10X Tris-EDTA Buffer by chelating divalent metal ions such as Mg²⁺ and Ca²⁺. These ions are essential cofactors for many nucleases that can degrade nucleic acids. By binding these ions, EDTA effectively protects DNA and RNA samples from enzymatic degradation during laboratory procedures, making it invaluable in protocols like DNA extraction and purification.

What industries commonly use 10X Tris-EDTA Buffer?

10X Tris-EDTA Buffer is widely used in various industries, including biotechnology, pharmaceuticals, and academic research. It is essential for laboratories engaged in genetic engineering, molecular diagnostics, and biochemical research. For example, it is a staple in assays performed in academic settings for teaching and experimental purposes, as well as routine quality control in manufacturing environments.

Can 10X Tris-EDTA Buffer be used with other buffers or solutions?

Yes, 10X Tris-EDTA Buffer can be used in combination with other buffers or solutions based on specific experimental requirements. However, it is essential to ensure compatibility, particularly in enzymatic reactions. For instance, when setting up a PCR reaction, it may be combined with other buffers that maintain pH and ionic strength, ultimately enhancing reaction efficiency and preventing degradation of nucleic acids.

Is 10X Tris-EDTA Buffer suitable for all DNA applications?

While 10X Tris-EDTA Buffer is suitable for a wide range of DNA applications, it's important to assess specific needs based on the procedure. For instance, in certain cases that require higher salt concentrations, additional reagents may be needed. However, generally, it serves well in common applications like DNA amplification, sequencing, and gel electrophoresis, alongside sample preparation.

Where can I buy 10X Tris-EDTA Buffer TE Buffer in Lithuania?

You can purchase 10X Tris-EDTA Buffer TE Buffer; 100mM Tris, 10mM EDTA, pH 8, 250 mL by Cepham Life Sciences through Ubuy. Ubuy is a reliable platform offering various scientific products, including laboratory reagents. By searching for the product on their website, you can easily find the necessary specifications and purchase details tailored to your needs.

Kaip pirkti 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM internetu iš Ubuy?

Lengva pirkti 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.

Ar 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM galima apsipirkti internetu Lithuania?

Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.

Kiek laiko užtrunka gauti prekę po užsakymo?

Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

Cepham Life Sciences Žr. originalą

10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM EDTA, pH 8), 250 mL- by Cepham Life Sciences

Cepham Life Sciences

Elementas #: 19753218

10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM EDTA, pH 8), 250 mL- by Cepham Life Sciences

Elementas #: 19753218

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What Stands Out

Precise pH Stability

Maintains a consistent pH of 8, ensuring optimal conditions for nucleic acid stability and reaction efficiency, crucial for accurate laboratory results.

High Concentration

Contains 100mM Tris and 10mM EDTA, providing enhanced chelation of metal ions, which protects DNA and RNA from degradation during experiments.

Convenient Volume

Offered in a 250 mL bottle for hassle-free usage, reducing the need for frequent restocking while supporting multiple laboratory applications effectively.

Produkto Aprašymas

Container Type	Bottle
Item Form	Liquid
Volume	250 ml
Brand Name	Cepham Life Sciences
Manufacturer	Cepham Life Sciences
Part Number	10421
Brand	Cepham Life Sciences
Item Volume	250 ml
Package Information	Bottle
Item Weight	0.6 lbs (270 grams)

Who Should Buy?

Molecular Biologists
Ideal for molecular biology experiments requiring precise buffer conditions for nucleic acid stabilization and storage.
Genetic Research
Useful for researchers involved in genetic analysis, DNA extraction, and PCR reaction setups.
Laboratories
Essential for various laboratory applications needing consistent pH and ion concentration in buffers.

Clinical Diagnostics
Not suitable for clinical diagnostic applications where strict regulatory compliance and specific formulations are required.

PRODUKTO APRAŠYMAS

10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM EDTA, pH 8), 250 mL- by Cepham Life Sciences

About This Item

Introducing our high-quality 10X Tris-EDTA Buffer TE Buffer by Cepham Life Sciences. This 250 mL bottle of concentrated TE (Tris-EDTA) Buffer is specifically designed to meet the needs of molecular biologists, researchers, and laboratory professionals. Designed with precision and expertise, our 10X Tris-EDTA Buffer TE Buffer provides a balanced and effective solution for a range of applications in molecular biology. With a pH of 8, this buffer contains 100mM Tris and 10mM EDTA, ensuring optimal performance in various experimental procedures. Not all TE buffers are created equal, but when you choose Cepham Life Sciences, you can trust that you are using a product of the highest quality.

Our TE buffer is manufactured under strict quality control measures to ensure its reliability and consistency. One of the key benefits of using Tris-EDTA Buffer in your experiments is its ability to stabilize DNA and RNA, making it an essential component in DNA extraction, PCR, and other molecular biology techniques. This buffer helps to maintain the integrity of nucleic acids, ensuring reliable and accurate results. Preparing TE Buffer solution is made easy with our concentrated 10X Tris-EDTA Buffer. Simply dilute it to the desired concentration with ultra-pure water, and you're ready to go.

Plus, with our generous 250 mL bottle, you'll have plenty of buffer solution to last through multiple experiments. Beyond its exceptional performance, our Tris-EDTA Buffer is also designed for convenience and ease of use. The sturdy bottle and secure seal ensure safe storage and handling, while the clear labeling provides all the necessary information you need for accurate preparation and usage. When it comes to buffer solutions, trust Cepham Life Sciences for top-quality products that deliver consistent and reliable results. Our TE Buffer is competitively priced, offering excellent value for money without compromising on quality. So, whether you're conducting DNA extractions, PCR, or any other molecular biology experiments, our 10X Tris-EDTA Buffer TE Buffer is the perfect choice.

Buy it online today and experience the convenience, reliability, and exceptional performance that Cepham Life Sciences is known for. Take your research to the next level with our TE Buffer solution.

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klausimas: Kaip pirkti 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM internetu iš Ubuy?

Answer: Lengva pirkti 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.
klausimas: Ar 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM galima apsipirkti internetu Lithuania?

Answer: Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.
klausimas: Kiek laiko užtrunka gauti prekę po užsakymo?

Answer: Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

Cepham Life Sciences Buffers Editorial Review

Cepham Life Sciences offers high-quality 10X Tris-EDTA Buffer TE Buffer, which comes in 250 mL bottles. The buffer has a pH of 8 and contains 100mM Tris and 10mM EDTA. The buffer ensures efficient nucleic acid protection in various applications and is perfect for DNA and RNA extraction, enzymatic reactions, and molecular biology research. It can also be used for gel electrophoresis, blotting, and hybridization. Customer Feedback: Customers are generally pleased with the performance of the Cepham Life Sciences 10X Tris-EDTA Buffer TE Buffer. Many have reported successful DNA and RNA extractions using this buffer, and it has also helped in achieving better results in gel electrophoresis experiments. Some have even noted that the buffer offers good value for money, as the 250 mL bottle can last for a long time.

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High-quality buffer
Efficient nucleic acid protection
suitable for various applications
Good value for money

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10X Tris-EDTA Buffer (TE Buffer); (100mM Tris, 10mM EDTA, pH 8), 250 mL- by Cepham Life Sciences

€ 62

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What Stands Out

Produkto Aprašymas

Who Should Buy?

PRODUKTO APRAŠYMAS

About This Item

Klientų klausimai ir atsakymai

Cepham Life Sciences Buffers Editorial Review

Customer Reviews & Ratings

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