What is 50X TAE Buffer Tris-Acetate-EDTA used for?

50X TAE Buffer Tris-Acetate-EDTA is primarily used in molecular biology for nucleic acid electrophoresis. This concentrated buffer allows for efficient separation of DNA and RNA in gel electrophoresis techniques. When diluted, it maintains the pH and ionic environment necessary for accurate results during DNA analysis, cloning, and other genetic applications.

How do you prepare 1X TAE Buffer from 50X concentration?

To prepare 1X TAE Buffer from 50X concentration, dilute the buffer by mixing 1 part of 50X TAE with 49 parts of distilled water. This results in a final volume where the buffer is at the appropriate dilution for running gel electrophoresis, ensuring the components are balanced for optimal performance in your experiments.

Why is Tris-Acetate-EDTA preferred over other buffers?

Tris-Acetate-EDTA (TAE) is favored for its ability to provide consistent buffering capacity and low electroendosmosis during electrophoresis. Unlike other buffers, TAE allows for the recovery of nucleic acids post-electrophoresis as it does not interfere with downstream applications, making it ideal for both analysis and cloning of DNA.

Can TAE Buffer be reused after gel electrophoresis?

Generally, TAE Buffer may be reused if it remains clean and free from contaminants. However, repeated use can compromise the buffer's pH and ionic strength, affecting the results of subsequent experiments. Always evaluate the quality of the buffer after each use and consider preparing fresh buffer for critical applications to maintain accuracy.

What are the storage conditions for 50X TAE Buffer?

50X TAE Buffer should be stored in a cool, dark place, ideally at room temperature or in a refrigerator to maintain its efficacy. Properly sealing the bottle after use will prevent contamination. If stored correctly, it can remain stable for an extended period, ensuring it's ready for your experimental needs.

Is 50X TAE Buffer compatible with all types of gel electrophoresis?

Yes, 50X TAE Buffer is compatible with various gel electrophoresis methods, including agarose and polyacrylamide gels. While TAE is typically used for DNA applications, it's important to adapt your experiments based on the specific type of nucleic acids being analyzed to achieve the best results.

How does the concentration of TAE Buffer impact electrophoresis results?

The concentration of TAE Buffer used directly impacts the resolution and migration speed of nucleic acids during electrophoresis. Higher concentrations can lead to slower migration and better resolution in separating fragments, while lower concentrations may generate faster migration, making it necessary to optimize based on the experiment's needs.

What pH does 50X TAE Buffer maintain?

50X TAE Buffer typically maintains a pH around 8.0, which is optimal for most enzymatic reactions and electrophoresis processes. This pH ensures stability and functionality, providing a conducive environment for nucleic acid interactions and separation during experimental procedures in molecular biology.

Can I use 50X TAE Buffer for RNA electrophoresis?

Yes, 50X TAE Buffer can be used for RNA electrophoresis, although many prefer using 1X TBE for RNA as it offers sharper bands. However, TAE still effectively provides a stable environment for RNA separation, making it a suitable choice in many laboratory applications involving RNA.

Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 100 mL in Lithuania?

You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 100 mL from Ubuy, which offers a wide selection of laboratory supplies that can be delivered to your location in Lithuania. Ubuy ensures that you receive a product that meets your experimental requirements.

Kaip pirkti 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL internetu iš Ubuy?

Lengva pirkti 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.

Ar 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL galima apsipirkti internetu Lithuania?

Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.

Kiek laiko užtrunka gauti prekę po užsakymo?

Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.

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50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

Elementas #: 85519151

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50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.

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What Stands Out

High Purity

Formulated with high-quality components, this TAE buffer ensures reliable results in your molecular biology experiments, minimizing contamination risks and enhancing reproducibility in gel electrophoresis.

Convenient Size

The 100 mL volume is ideal for lab use, providing ample buffer for multiple experiments while reducing waste, enabling efficient resource management for researchers in DNA and RNA analysis.

Ready-to-Use

This pre-prepared 50X TAE buffer allows for immediate application in your procedures, saving time in preparation and ensuring that your experiments can proceed without unnecessary delays.

Produkto Aprašymas

Container Type	Bottle
Item Form	Gel
Item Weight	4 ounces (113.4 grams)
Volume	100 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.17
Brand	eCon Lab Supply
Item Volume	100 ml
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for DNA electrophoresis, providing efficient buffering capacity for nucleic acid analysis in lab experiments.
Academic Institutions
Supports educational labs where students learn about genetic analysis, making it a staple in biology curricula.
Research Facilities
Highly suitable for ongoing research projects involving DNA and RNA studies, thanks to its reliable buffering properties.

Non-Laboratory Use
Not designed for applications outside of laboratory settings; unsuitable for general household or consumer use.

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50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

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klausimas: Kaip pirkti 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL internetu iš Ubuy?

Answer: Lengva pirkti 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.
klausimas: Ar 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL galima apsipirkti internetu Lithuania?

Answer: Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.
klausimas: Kiek laiko užtrunka gauti prekę po užsakymo?

Answer: Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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High-quality buffer solution
Stable and reliable performance
Easy to use in experiments
Excellent for electrophoresis
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Some may find the size too small.

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Savybės ir privalumai

Ideal for running electrophoresis of nucleic acids
Provides electrical conductivity and maintains pH
Protects DNA from nucleases
Suitable for both genomic and large supercoiled DNA
Can be used as a gel preparation buffer

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50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

€ 42

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What Stands Out

Produkto Aprašymas

Who Should Buy?

PRODUKTO APRAŠYMAS

Klientų klausimai ir atsakymai

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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€ 42

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