What is NP40 Cell Lysis Buffer and how is it used?

NP40 Cell Lysis Buffer is a specialized solution designed for the efficient lysis of cells to extract cellular components such as proteins and nucleic acids. It operates at a neutral pH of 7.4 and is recommended for use in cell biology experiments where high yield and purity are critical. This buffer can disrupt both cell membranes and nuclear membranes, effectively releasing contents for further analysis. It is widely used in research laboratories for applications like Western blotting, immunoprecipitation, and enzyme assays.

What are the key components of NP40 Cell Lysis Buffer?

The NP40 Cell Lysis Buffer contains Nonidet P-40 (NP-40), a non-ionic detergent, along with salts and buffering agents that maintain the pH at 7.4. These components work synergistically to solubilize membrane proteins without denaturing them, which is crucial for downstream applications like SDS-PAGE or mass spectrometry. The balance of these ingredients is tailored to provide optimal lysis conditions while preserving protein functionality, making it a popular choice for life science research.

Can NP40 Cell Lysis Buffer be used for different cell types?

Yes, NP40 Cell Lysis Buffer is versatile and can be applied to various cell types, including mammalian, yeast, and bacterial cells. Its formulation allows for efficient lysis regardless of the cellular composition. This makes it an excellent tool in diverse experimental setups, such as studying protein interactions in mammalian cells or extracting plasmids from bacterial cells. However, it's recommended to optimize conditions based on specific cell types and experimental goals.

How should NP40 Cell Lysis Buffer be stored?

NP40 Cell Lysis Buffer should be stored at 4°C to maintain its stability and effectiveness. For longer storage, aliquoting the buffer into smaller tubes can help minimize repeated freeze-thaw cycles, which may compromise its integrity. Always check for any signs of precipitation or discoloration before use. Proper storage extends its viability, ensuring consistent performance in experiments over time, especially in high-throughput applications.

What precautions should be taken while using NP40 Cell Lysis Buffer?

When using NP40 Cell Lysis Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye contact with the buffer. Additionally, work in a well-ventilated area to avoid inhaling vapors. Ensure that all reagents are at the correct temperature prior to use and that your lab solutions are prepared under sterile conditions to prevent contamination. These precautions ensure a safe and controlled experimental environment.

How does NP40 Cell Lysis Buffer affect protein assays?

NP40 Cell Lysis Buffer is designed to maintain protein stability while extracting them from cells. It minimizes protein denaturation, which is crucial for assays such as Western blotting or ELISA. This buffer allows for the recovery of functional proteins, making it highly suitable for assays where protein activity is determined. Users often find that using this buffer leads to more consistent and reproducible results in their analyses.

Is NP40 Cell Lysis Buffer suitable for DNA extraction?

While NP40 Cell Lysis Buffer is primarily developed for protein extraction, it can also aid in DNA extraction by breaking down cell membranes that house both proteins and nucleic acids. However, to ensure purity, it's advisable to perform a phenol-chloroform extraction following lysis. This approach allows for the recovery of high-quality DNA suitable for applications such as PCR. Researchers often use this buffer to facilitate simultaneous protein and DNA study within the same sample.

Are there any alternatives to NP40 Cell Lysis Buffer?

Yes, there are several alternatives to NP40 Cell Lysis Buffer, including Triton X-100 and SDS-based lysis buffers. Each of these alternatives may have specific advantages depending on the application. For instance, Triton X-100 is milder and less likely to disrupt protein structure, making it suitable for applications requiring intact proteins. In contrast, SDS is more effective for complete denaturation of proteins. Researchers often choose a buffer based on their specific experimental needs and the type of analysis they plan to perform.

How much NP40 Cell Lysis Buffer should I use per sample?

The optimal volume of NP40 Cell Lysis Buffer typically depends on the type and number of cells being lysed. A general recommendation is to use 100-300 µL of the buffer per 1-10 million cells. Adjusting the volume may be necessary based on cell density and the specific requirements of your assay. Researchers find that using the right amount ensures efficient lysis while preventing the dilution of key cellular components, leading to more reliable experimental outcomes.

Where can I buy NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL in Lithuania?

You can purchase NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL from Ubuy in Lithuania. Ubuy offers a reliable platform for sourcing laboratory reagents and supplies, making it a convenient choice for researchers and scientists needing high-quality buffers and reagents. Ensure you check their website for availability and details on your specific needs.

Kaip pirkti NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL internetu iš Ubuy?

Lengva pirkti NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.

Ar NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL galima apsipirkti internetu Lithuania?

Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.

Kiek laiko užtrunka gauti prekę po užsakymo?

Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL

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Elementas #: 145284746

NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL

Elementas #: 145284746

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What Stands Out

Dual Concentration

The NP40 Cell Lysis Buffer is formulated at a 2X concentration, ensuring efficient and effective cell lysis in fewer steps, saving time for researchers while maintaining optimal results.

pH Stability

Maintaining a pH of 7.4, this buffer provides a controlled environment, crucial for preserving protein integrity during lysis, making it ideal for sensitive experiments and applications.

Generous Volume

With 250mL of solution, there is ample buffer for numerous applications, reducing the frequency of repurchasing and ensuring that labs can perform multiple experiments seamlessly.

Produkto Aprašymas

Container Type	Bottle
Item Form	Liquid
Item Weight	4 ounces (113.4 grams)
Volume	250 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.112
Brand	eCon Lab Supply
Item Volume	250 ml
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for extracting proteins from cells to facilitate downstream molecular biology applications.
Biochemistry Laboratories
Useful in enzymatic studies where cell lysis is needed to release cellular components.
Research Institutions
Beneficial for researchers conducting studies on cellular processes and protein interactions.

General Consumers
Not suitable for non-laboratory individuals due to specialized usage and safety considerations.

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NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL

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klausimas: Kaip pirkti NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL internetu iš Ubuy?

Answer: Lengva pirkti NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.
klausimas: Ar NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL galima apsipirkti internetu Lithuania?

Answer: Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.
klausimas: Kiek laiko užtrunka gauti prekę po užsakymo?

Answer: Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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Comprehensive range of biological buffers.
Ideal for research, diagnostics, drug development, and manufacturing.
Certified facility guarantees top-tier quality.
Designed for protein research and molecular biology.
NP40 Cell Lysis Buffer is suitable for key assays like ELISA and Western blotting.
Strict adherence to Good Manufacturing Practices ensures reliability.

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NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL

€ 61

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Produkto Aprašymas

Who Should Buy?

PRODUKTO APRAŠYMAS

Klientų klausimai ir atsakymai

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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€ 61

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