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Elementas #:
97964044
50X TAE Buffer, 100 mL
Elementas #:
97964044
€ 42
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50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.
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What Stands Out
Concentrated Solution
This 50X TAE buffer provides a highly concentrated formulation, ensuring ease of use and longer shelf life, making it ideal for high-throughput laboratories and educational institutions.
Versatile Applications
Designed for various molecular biology techniques, this buffer ensures optimal performance for DNA electrophoresis and other applications, effectively meeting diverse research needs in a single product.
Precise Composition
Formulated with high purity components, this TAE buffer guarantees reproducibility and reliability in experiments, catering to both novice and expert researchers who demand consistent results.
Produkto Aprašymas
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- 50X TAE (Tris-Acetate-EDTA) Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH. The EDTA present in the buffer inhibits metal dependent nucleases by chelating the divalent cations (Ca2+, Mg2+), and thereby protects the DNA from nucleases during the electrophoresis. The buffer may be used for both genomic and large supercoiled DNA, and also be as a gel preparation buffer.
| Item Form | Gel |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 100 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.171 |
| Included Components | Bottle |
| Brand | eCon-Lab-Supply |
| Item Volume | 100 Milliliters |
What's in the box
Bottle
Who Should Buy?
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Molecular Biologists
Ideal for electrophoresis applications, aiding in the separation and analysis of nucleic acids in a lab environment.
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Academic Researchers
Perfect for students and researchers conducting experiments that require buffer solutions in their molecular biology studies.
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DNA & RNA Studies
Essential for protocols involving DNA and RNA handling, ensuring stability and integrity during electrophoresis experiments.
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Clinical Laboratories
Not suitable for clinical environments where strict regulatory compliance and high precision are required in diagnostics.
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Undergraduate Courses
Beginner students may find ease of use with simpler buffers; TAE may be too complex for introductory labs.
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Non-Biological Users
Individuals not engaged in biological research or laboratory work would not need this specialized buffer solution.
PRODUKTO APRAŠYMAS
Turite klausimų? Kalbekites su mumis
Klientų klausimai ir atsakymai
-
klausimas:
What is 50X TAE Buffer used for?
Answer: 50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis. -
klausimas:
How do I dilute 50X TAE Buffer for use?
Answer: To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis. -
klausimas:
What is the shelf life of 50X TAE Buffer?
Answer: The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure. -
klausimas:
Is 50X TAE Buffer compatible with agarose gel electrophoresis?
Answer: Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands. -
klausimas:
Can I use 50X TAE Buffer for RNA electrophoresis?
Answer: Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results. -
klausimas:
What are the components of 50X TAE Buffer?
Answer: 50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments. -
klausimas:
Is 50X TAE Buffer necessary for DNA gel extraction?
Answer: 50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures. -
klausimas:
How should I store 50X TAE Buffer?
Answer: To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis. -
klausimas:
What are some common applications for using 50X TAE Buffer?
Answer: 50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results. -
klausimas:
Where can I buy 50X TAE Buffer, 100 mL?
Answer: You can buy 50X TAE Buffer, 100 mL on Ubuy in Lithuania. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.
eCon Lab Supply Buffers Editorial Review
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Argumentai "už"
- High purity formula
- Convenient 100 mL size
- Well-suited for various applications
- Easy to store and use
- Reliable performance
Minusai
- Cap can be slightly hard to open.
Product Price History
Svarbi informacija
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€ 42
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Savybės ir privalumai
- Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
- Inhibits nucleases to protect DNA integrity during experiments.
- Powers efficient running of both genomic and supercoiled DNA.
- Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
- Manufactured under strict quality control in an ISO certified facility.
- Ideal for research purposes, compliant with Good Manufacturing Practices.