What is 10x PBS Buffer and what is it used for?

10x PBS (Phosphate-Buffered Saline) Buffer is a saline solution that is commonly used in biological research and laboratory settings. This buffer helps maintain a stable pH and osmotic balance in biological samples, making it essential for various applications like cell culture, protein purification, and immunoassays. The '10x' indicates it's a concentrated form, which can be diluted to 1x for use. It's important for maintaining cell health and facilitating many laboratory protocols.

How do you prepare 1x PBS from 10x PBS Buffer?

To prepare a 1x PBS from a 10x PBS Buffer, simply dilute it with distilled water. For every 1 part of 10x PBS, add 9 parts of distilled water. This results in a final solution that is isotonic and pH-stable, ideal for various biological applications. For example, if you need 100 mL of 1x PBS, mix 10 mL of 10x PBS with 90 mL of distilled water. Accurate dilution ensures that your experimental results are reliable.

What are the benefits of using PBS Buffer in cell culture?

PBS Buffer is widely used in cell culture as it provides a stable osmotic environment and maintains pH levels, critical for cell survival and function. It acts as a transport medium for cells during procedures such as washing, diluting, or transferring cells. Additionally, PBS contains salts that help in maintaining the ionic strength necessary for cellular activities. For cell biologists, using PBS ensures that their results related to cell behaviors are consistent and reproducible.

Is 10x PBS Buffer suitable for use in immunoassays?

Yes, 10x PBS Buffer is highly suitable for use in immunoassays, including ELISA and western blotting. Its composition helps to prevent non-specific binding of antibodies, thus enhancing the accuracy of the assay results. The buffer maintains a stable pH, which is crucial during the reaction steps. For researchers, using PBS ensures that their assays yield high sensitivity and specificity, leading to reliable data.

Can 10x PBS Buffer be used in molecular biology applications?

Absolutely! 10x PBS Buffer is widely utilized in molecular biology applications, such as DNA extraction, RNA handling, and PCR preparation. Its isotonic nature helps to protect nucleic acids from degradation while facilitating hybridization processes. In experiments where you're working with sensitive nucleic acids, using PBS can enhance the yield and integrity, ensuring optimal performance in downstream applications.

What storage conditions are recommended for 10x PBS Buffer?

10x PBS Buffer should be stored at room temperature in a cool, dry place, away from direct sunlight. When properly stored, it can remain stable for extended periods. For longer-term storage, some labs prefer to keep it refrigerated. Always ensure to label your buffer correctly and check for any precipitates before use. Proper storage helps maintain the integrity of the buffer, ensuring reliable results in your experiments.

What is the pH level of 10x PBS Buffer and why is it important?

The typical pH level of 10x PBS Buffer is around 7.4, which is physiologically relevant for most biological samples. This neutral pH is crucial for maintaining proper cellular functions and conducting various experiments without causing cellular stress. Buffers like PBS play a vital role in keeping the pH stable during reactions, especially in biological assays. Therefore, ensuring the right pH helps yield consistent and reproducible experimental results.

Can you use 10x PBS Buffer for protein assays?

Yes, 10x PBS Buffer is commonly used in protein assays such as western blots and protein quantification. Its role includes diluting samples and washing steps, where maintaining a suitable environment is crucial to preserve protein integrity and functionality. For example, buffers like PBS help minimize protein denaturation and non-specific binding, which can lead to more precise and accurate assay results.

How long can PBS Buffer be used after opening?

Once opened, 10x PBS Buffer should ideally be used within a few months to ensure its effectiveness. However, if stored correctly—in a tightly sealed container, protected from contamination, and under appropriate conditions—it can last longer. Always check for clarity and the absence of precipitates before use. Monitoring the buffer's condition ensures that your experimental procedures remain reliable and that your results are valid.

Where can I buy 10x PBS Buffer 4 L in Lithuania?

You can purchase 10x PBS Buffer 4 L from Ubuy. They offer a wide range of laboratory supplies, including buffers commonly used for biological research. With Ubuy, you can find reliable products that are essential for your experiments, ensuring a smooth workflow in your laboratory settings.

Kaip pirkti 10x PBS Buffer (4 L) internetu iš Ubuy?

Lengva pirkti 10x PBS Buffer (4 L) internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.

Ar 10x PBS Buffer (4 L) galima apsipirkti internetu Lithuania?

Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 10x PBS Buffer (4 L) nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.

Kiek laiko užtrunka gauti prekę po užsakymo?

Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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Each 10X PBS solution is ready to use upon dilution to the desired concentration.

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10x PBS Buffer (4 L)

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High Concentration

The 10x PBS buffer offers a highly concentrated solution suitable for various laboratory applications, ensuring enhanced performance and reliability compared to standard formulations.

Large Volume

With 4 liters of buffer, this product is ideal for larger scale experiments, reducing the frequency of replenishment and saving time in busy lab environments.

Versatile Use

Designed for multiple applications including cell culture and protein work, this buffer meets diverse research needs, making it a valuable asset for any scientific project.

Produkto Aprašymas

Brand Name	Generic
Manufacturer	Bioland Scientific LLC
Included Components	buffer
Model Number	PBS01-4L
Part Number	PBS01-4L
Item Weight	8.8 lbs (3.99 kg)

Who Should Buy?

Laboratories
Ideal for research laboratories conducting experiments requiring consistent and reliable buffer solutions for biochemistry applications.
Cell Culture
Perfect for cell culture applications, providing the necessary osmotic balance and pH stability for growing cells.
Molecular Biology
Useful for molecular biology techniques, such as DNA/RNA extraction, where a buffer system is crucial for sample integrity.

General Consumers
Not suitable for general consumers with no background in scientific research or laboratory practices.

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10x PBS Buffer (4 L)

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klausimas: Kaip pirkti 10x PBS Buffer (4 L) internetu iš Ubuy?

Answer: Lengva pirkti 10x PBS Buffer (4 L) internetu iš Ubuy.. Jums tereikia ieškoti prekės, išsiregistruodami pasirinkti pristatymo būdą ir gauti pristatymą į jūsų vietą.
klausimas: Ar 10x PBS Buffer (4 L) galima apsipirkti internetu Lithuania?

Answer: Taip, „Ubuy Lithuania“ šį produktą galite įsigyti už priimtiną kainą.. 10x PBS Buffer (4 L) nepasiekiamas vietoje, bet galite patikėti mums greitojo siuntimo paslaugas.
klausimas: Kiek laiko užtrunka gauti prekę po užsakymo?

Answer: Užsakytos prekės pristatymo laikas priklauso nuo to, ką užsisakėte ir pasirinktą pristatymo būdą.. Numatomas pristatymo laikas nurodomas atsiskaitymo metu, todėl apsipirkdami būkite nerūpestingi.

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Highly effective for experiments
Stable and long-lasting
Easy to use and handle
consistent results across tests
Suitable for various applications

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Labeling could be clearer.

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Savybės ir privalumai

Ready-to-use 10X PBS solution for convenience.
Easy dilution to achieve desired concentration.
Ideal for applications like Western blot and ELISA.
Maintains a stable pH of 7.4 ± 0.2.
Composed of ultrapure-grade phosphate buffers.
Filtered for quality and reliability.

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10x PBS Buffer (4 L)

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